Keywords
wound healing
analgesic activity
regenerative properties
topical formulation
Abstract
Purulent wound is one of the thorniest, difficult and urgent issues in clinical practice. Due to the start of full-scale military actions in Ukraine in 2022, the number of purulent-necrotic wounds has increased several times, almost 40% of all wounds are purulent-necrotic. Purulent-inflammatory wounds are very acute and often lead to generalized infections, the development of sepsis and also the death of patients. Thus, the development of new anti-inflammatory and antioxidant drugs in the form of soft dosage forms is relevant today. The aim of the study was to investigate regenerative and analgesic effects of a novel anhydrous gel "Hypericum-Derm" for treatment of wounds for I and II phase.
The wound-healing effect of anhydrous gel "Hypericum-Derm" was studied on a model of a full-layer stencil wound. The first group of rats was a control pathology (the animals' stencil wound was treated with a hydrogen peroxide solution), the second group was a polyethylene glycol gel 400/1500 (8:2) base without active pharmaceutical ingredients, the third group was "Levomekol" ointment, the fourth group was "Wundehil" ointment, the fifth group was anhydrous gel "Hypericum-Derm". To measure the pain thresh- old, the hot plate test was chosen. In the experiments were used 4 groups of animals (7 mice each):
1 group – control group, animals without the use of gel; 2 group – animals, to whom polyethylene glycol gel 400/1500 (8 : 2) base was applied; 3 group – animals, which were applied the comparison drug –2% lidocaine hydrochloride gel; 4 group – animals with application "Hypericum-Derm" gel. For the "hot plate" test gels were applied 20 min before the corresponding irritation to all limbs of the mice. The wound healing rate when using the anhydrous gel "Hypericum-Derm" was 55 and 51% higher on days 5 and 8 compared to the control group. Complete wound healing was on day 12 when using the anhy- drous gel "Hypericum-Derm", and in the case of control pathology on day 21. It was established that the investigated anhydrous gel "Hypericum-Derm" reliably increases the latent period of the manifestation of pain on a hot surface by 3 times relative to the control values. It was established that anhydrous gel "Hypericum-Derm" possessed wound-healing and analgesic effects.
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